Journal: Stem Cell Research & Therapy

Article Title: hAMSCs regulate EMT in the progression of experimental pulmonary fibrosis through delivering miR-181a-5p targeting TGFBR1

doi: 10.1186/s13287-024-04095-3

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Article Snippet: Anti-Collagen Type I/COL1A1 Antibody , BOSTER BA0325.

Techniques: Isolation, Reverse Transcription, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Nature Communications

Article Title: Spatially organized tumor-stroma boundary determines the efficacy of immunotherapy in colorectal cancer patients

doi: 10.1038/s41467-024-54710-3

Figure Lengend Snippet: a Bubble plots represent the expression profiling of PDCD1 and CD274 in single cell clusters from scRNA-seq dataset. The plots are sized by the fraction of cells with positive gene expression, while the color represents the gene expression level . b The interactions among indicated cell clusters through PD1-PD-L1 axis from scRNA-seq dataset. c The box and whisker plot shows show the nearest distance from DC_ LAMP3 to CD4_ CXCL13 (N number of the counted cell-cell distances: distant stroma =918, boundary region=1867; tumor center=251) or ( d ) to CD8_ CXCL13 (N number of the counted cell-cell distances: distant stroma =368, boundary region=567; tumor center=45) in distance stroma, tumor-stroma boundary and tumor center from treatment naïve dMMR patients. Data are represented as mean±IQR and analyzed by unpaired 2-tailed Student t test. ns, not significant; *, p < 0.05; **, p < 0.01. Pie charts represent the fractions of CD4_ CXCL13 or CD8_ CXCL13 in the indicated regions stratified by colors in accordance with ( c ) d . Data are analyzed by unpaired Student-t test. ns, not significant; ****, p < 0.0001. e Representative mIF images of panCK, COL1A1, PD-L1 and PD1 in treatment naïve dMMR patients (5 samples were analyzed). DAPI was used as a positive control for cell nuclei staining. Scale bars were set as 5 mm for global image (left) and 50μm for FOVs (middle and right). f The box and whisker plot shows the nearest distances from PD1 + cells to PDL1 + cells in distance stroma, tumor-stroma boundary and tumor center from 7 treatment naïve dMMR patients. Each dot represents a PD1-expressing cell. N number of the counted cell-cell distances: stroma = 750; boundary = 935; tumor = 892. Data are represented as mean ± IQR and analyzed by unpaired 2-tailed Student t test with Bonferroni correction. Ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ***, p < 0.0001. g The box and whisker plot shows the T cell exhaustion scores in the distant stroma, tumor-stroma boundary and tumor center from treatment naïve dMMR patients. Data are represented as mean±IQR and analyzed by unpaired 2-tailed Student t test. Ns not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ***, p < 0.0001. N number of the spots: distant stroma =86, boundary region=12; tumor center=287. h Representative images of H&E staining, FOV, and spatial map of T cell exhaustion scores in the tumor-stroma boundary of the treatment naïve dMMR patient #56. Scale bars were set as 1.5 mm for global image (left) and 500 μm for FOVs . i Heatmap of PDCD1 and CD274 expressions in the distant stroma, tumor-stroma boundary and tumor center of treatment naïve dMMR patients.

Article Snippet: Anti-COL1A1 rabbit mAb , 1:500 , Cell Signaling , CST72026S.

Techniques: Expressing, Gene Expression, Whisker Assay, Positive Control, Staining

Journal: Nature Communications

Article Title: Spatially organized tumor-stroma boundary determines the efficacy of immunotherapy in colorectal cancer patients

doi: 10.1038/s41467-024-54710-3

Figure Lengend Snippet: a The top 5 GO terms of DEGs (p value < 0.001; fold change >2) identified from CAF_ CXCL14 and CAF_ CXCL8 clusters are shown. Comparison is made by two-tailed t test. b GSEA plot of the upregulated genes related to extracellular matrix organization in CAF_ CXCL14 (n cells=268) compared to CAF_ CXCL8 ( n cells=118) is shown. Comparison is made by two-tailed t test. c RCTD frequencies of CAF_ CXCL14 (up) and ECM organization signature scores (below) in the tumor-stroma boundary of dPR/dCR (n spots=1436) and dSD (n spots=2244) patients are shown as violim plots. Data are analyzed by unpaired 2-tailed Student t test. Ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ***, p < 0.0001. d Pearson correlation of RCTD frequencies of CAF_ CXCL14 and ECM organization signature scores in dPR/dCR and dSD patients. e Loess smoothed curves and ( f ) violin plots of RCTD frequencies of CAF_ CXCL14 (up) and ECM organization signature scores (below) in tumor ( + 300 μm, n spots = 9313) to the tumor-stroma boundary (0 μm, n spots = 56,357) from treatment naïve pMMR and dSD patients are shown. The tumor edge region ( + 150 μm to 0 μm) is highlighted in the yellow frame. g Representative images of FOV, CAF_ CXCL14 and ECM organization scores in the tumor-stroma boundary of treatment naïve pMMR patient #59 and dSD patient #95 are shown. h Representative images of Masson’s trichrome staining from treatment naïve dMMR and pMMR patients (3 samples were analyzed in each group). Scale bar = 50 μm. i Representative mIF images of panCK, COL1A1, CD3 and CXCL14 in indicated patient groups (3 samples were analyzed in each group). DAPI was used as a positive control for cell nuclei staining. Scale bars, 50 μm. j CXCL14 expression level and ECM organization scores in fibroblasts from the three public scRNAseq datasets , , .

Article Snippet: Anti-COL1A1 rabbit mAb , 1:500 , Cell Signaling , CST72026S.

Techniques: Comparison, Two Tailed Test, Staining, Positive Control, Expressing

Journal: Nature Communications

Article Title: Spatially organized tumor-stroma boundary determines the efficacy of immunotherapy in colorectal cancer patients

doi: 10.1038/s41467-024-54710-3

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Article Snippet: Anti-COL1A1 rabbit mAb , 1:500 , Cell Signaling , CST72026S.

Techniques: